Scientific Results
Agents tested
We have tested the reliability of the FCMA on a number of different
chemical and physical agents, including well known mutagens as well
as negative controls and false negatives (known mutagens that test
negative in the MLA). As shown in the Table, the FCMA reliably
measures mutations from a wide variety of agents that cause DNA
damage in different ways. These include DNA binding drugs, point
mutagens, oxidizing agents or reactive oxygen species (ROS), DNA
cross linking agents, heavy metals, agents that cause DNA adducts,
and clastogens (agents that cause large deletions and chromosomal
aberrations). Different agents vary in their degree of mutagenicity,
ranging from weakly mutagenic to strongly mutagenic.
Relative Sensitivity
The mutation yield for various doses of ionizing radiation is shown in
Figure 1. For comparison, published results using the standard
mouse lymphoma assay (MLA) are also shown. It is clear that the
FCMA is about 6-fold more sensitive than the MLA. This means that
significant mutations can be measured from lower doses of a test
compound, thus making the FCMA more reliable in testing drugs for
mutagenesis.
Publications
Ross CD, Lim C-U, Fox MH. Assay to measure CD59 mutations in
CHO Al cells using flow cytometry. Cytometry 66A:85-90, 2005.
French CT, Ross CD, Keysar SB, Joshi DD, Lim C-U, Fox MH.
Comparison of the mutagenic potential of 17 physical and chemical
agents analyzed by the flow cytometry mutation assay. Mutation
Research 602:14-25, 2006.
Ross CD, French CT, Keysar SB, Fox MH: Mutant spectra of irradiated
CHO A(L) cells determined with multiple markers analyzed by flow
cytometry. Mutation Research 2007 (Epub, in press).
Agents tested
We have tested the reliability of the FCMA on a number of different
chemical and physical agents, including well known mutagens as well
as negative controls and false negatives (known mutagens that test
negative in the MLA). As shown in the Table, the FCMA reliably
measures mutations from a wide variety of agents that cause DNA
damage in different ways. These include DNA binding drugs, point
mutagens, oxidizing agents or reactive oxygen species (ROS), DNA
cross linking agents, heavy metals, agents that cause DNA adducts,
and clastogens (agents that cause large deletions and chromosomal
aberrations). Different agents vary in their degree of mutagenicity,
ranging from weakly mutagenic to strongly mutagenic.
Relative Sensitivity
The mutation yield for various doses of ionizing radiation is shown in
Figure 1. For comparison, published results using the standard
mouse lymphoma assay (MLA) are also shown. It is clear that the
FCMA is about 6-fold more sensitive than the MLA. This means that
significant mutations can be measured from lower doses of a test
compound, thus making the FCMA more reliable in testing drugs for
mutagenesis.
Publications
Ross CD, Lim C-U, Fox MH. Assay to measure CD59 mutations in
CHO Al cells using flow cytometry. Cytometry 66A:85-90, 2005.
French CT, Ross CD, Keysar SB, Joshi DD, Lim C-U, Fox MH.
Comparison of the mutagenic potential of 17 physical and chemical
agents analyzed by the flow cytometry mutation assay. Mutation
Research 602:14-25, 2006.
Ross CD, French CT, Keysar SB, Fox MH: Mutant spectra of irradiated
CHO A(L) cells determined with multiple markers analyzed by flow
cytometry. Mutation Research 2007 (Epub, in press).
| Cytomation GTX, Inc. |
| Flow cytometry histogram of the mutants and non-mutants |
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